Abstract
Background
Xenogenic organ replacement from pig to human is a possibility provided sterility and immunogenicity are taken care of. The objective of this study was to check the sterility of processed porcine pulmonary xenografts to mark them ‘safe’ for clinical application, without the threat for xenozoonoses.
Methods
A total of 148 porcine pulmonary valve conduits were screened pre and post processing for bacterial, fungal and viral contamination. The bacterial and fungal contaminants such as Candida species, filamentous fungus, yeast like organisms, gram negative bacteria and porcine endogenous retrovirus (PERV) were isolated from the preprocessed porcine conduits. These harvested conduits were collected in a cocktail of antimicrobials and Hanks balanced salt solution. Next, they were subjected to ‘processing’ i.e., made completely acellular, strong and biocompatible in all respects. Finally, sterility checks were carried out for bacterial, fungal and viral (PERV) contamination.
Result
Microbial evaluation performed in this study revealed that the porcine pulmonary conduits were made ‘microbe free’ on subjecting to the special staged decellularization technique.
Conclusion
The porcine xenograft conduit processed in the laboratory, which has an added advantage of numbers and sizes, can replace the gold standard homograft safely.
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Balasundari, R., Gupta, R., Sivasubramanian, V. et al. Complete microbe free processed porcine xenograft for clinical use. Indian J Thorac Cardiovasc Surg 23, 240–245 (2007). https://doi.org/10.1007/s12055-007-0049-y
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DOI: https://doi.org/10.1007/s12055-007-0049-y